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Antibodies
Anti-KMT4 / Dot1L antibody
Anti-KMT4 / Dot1L antibody from Abcam
Be the first to write a review!
Citations:
(15)
Supplier Page
Supplier Page from
Abcam for
Anti-KMT4 / Dot1L antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-KMT4 / Dot1L antibody
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab72454
Antibody Type
Primary
Quantity
100 UL
Host
Rabbit
Conjugate/Tag
Unconjugated
Clonality
Polyclonal
Isotype
IgG
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunoprecipitation, Western blot
Concentration
0.2 mg/ml
Research Area
Epigenetics
Gene Name
DOT1L
Reactivity
Human
Target
KMT4 / Dot1L
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (15)
(1)
Combinatorial targeting of a chromatin complex comprising Dot1L, menin and the tyrosine kinase BAZ1B reveals a new therapeutic vulnerability of endocrine therapy-resistant breast cancer
Breast Cancer Research
December 1, 2022
Annamaria Salvati, Viola Melone, Assunta Sellitto, Francesca Rizzo, Roberta Tarallo, Tuula A. Nyman, Giorgio Giurato, Giovanni Nassa, Alessandro Weisz
C-terminal (F-10
sc-8002
,
Santa Cruz Biotechnology
, Dallas, Texas), anti-ERα (
ab3575
) and
anti-KMT4/Dot1L
(
ab72454
) from
Abcam
(Cambridge, UK), anti-
Dot1L
(
A300-953A
), antimenin (A300–105 A) and anti-WSTF (BAZ1B;
A300-446A
)
(2)
Combinatorial targeting of menin and the histone methyltransferase DOT1L as a novel therapeutic strategy for treatment of chemotherapy-resistant ovarian cancer
Cancer Cell Int
November 4, 2022
Elena Alexandrova, Jessica Lamberti, Domenico Memoli, Claudia Quercia, Viola Melone, Francesca Rizzo, Roberta Tarallo, Giorgio Giurato, Giovanni Nassa, Alessandro Weisz
Louis, MO); rabbit polyclonal anti-menin (A300-105A, Thermo Fisher Scientific) and
anti-KMT4/Dot1L
(
ab72454
,
Abcam
, Cambridge, UK). Total RNA extraction Total RNA was extracted from all cell lines using
TRIzol reagent
(3)
A novel screening strategy to identify histone methyltransferase inhibitors reveals a crosstalk between DOT1L and CARM1†
RSC Chemical Biology
April 6, 2022
Yang Si et al.
overnight at 4 °C with the
CARM1
antibody (
Abcam
ab245466
)
and SDS–PAGE analysis
for DOT1L antibody (
Abcam
ab72454
) and
CARM1
antibody (Abcam
ab245466
). Histone marks pulldown Exponentially growing U2OS cells were
(4)
Histone methyltransferase Dot1L inhibits pancreatic cancer cell apoptosis by promoting NUPR1 expression
Journal of International Medical Research
March 30, 2022
Lin Shan, Chen Hao, Zheng Jun, Cai Qinghe
with specific antibodies in 5% bovine serum albumin. The antibodies used were as follows: anti-Dot1L (
ab72454
,
Abcam
, Cambridge, UK), anti-NUPR1 (
ab161980
,
Abcam
), anti-GAPDH (
sc-20357
,
Santa Cruz Biotechnology
,
(5)
Inhibition of the SUV4-20 H1 histone methyltransferase increases frataxin expression in Friedreich's ataxia patient cells
Journal of Biological Chemistry
December 25, 2020
Gabriela Vilema-Enríquez et al.
anti-EZH2 (Cell Signaling, catalog no. 5246 1/2500), rabbit polyclonal anti-DOT1L (
Abcam
, catalog no.
ab72454
, 1/1000), rabbit polyclonal anti-H4K20me1 (
Abcam
, catalog no.
ab9051
, 1/500), rabbit polyclonal anti-H4K20me2
(6)
Histone methyltransferase DOT1L coordinates AR and MYC stability in prostate cancer
Nature Communications
December 1, 2020
R. Vatapalli et al.
(
ab1791
;
Abcam
,
1:3000
), GAPDH (
sc-20357
;
Santa Cruz
Biotech,
1:1000
), DOT1L (EMD Millipore MABE425,
Abcam
ab72454
,
1:100
),
MYC
(
Abcam
ab32072
,
1:1000
), Halotag (
Promega
G9211
,
1:1000
), Flag (
Sigma-Aldrich
F1804
,
1:1000
),
(7)
The Histone Methyltransferase DOT1L Is a Functional Component of Estrogen Receptor Alpha Signaling in Ovarian Cancer Cells
Cancers (Basel)
November 4, 2019
Annamaria Salvati, Valerio Gigantino, Giovanni Nassa, Giorgio Giurato, Elena Alexandrova, Francesca Rizzo, Roberta Tarallo, Alessandro Weisz
Bethyl Laboratories
, Montgomery, Alabama), β-actin (
A1978
,
Sigma Aldrich
, Milan, Italy), Rabbit
anti-KMT4/DOT1L
(
ab72454
), anti-Histone H3, total, (
ab1791
), anti-H3K79me1 (
ab2886
), anti-H3k79me2 (
ab3594
), anti-H3K79me3 (
ab2621
),
(8)
Inhibition of histone methyltransferase DOT1L silences ERα gene and blocks proliferation of antiestrogen-resistant breast cancer cells
Science advances
February 1, 2019
Nassa G, Salvati A, Tarallo R, Gigantino V, Alexandrova E, Memoli D, Sellitto A, Rizzo F, Malanga D, Mirante T,
et al
.
Bethyl Laboratories
), β-actin (
A1978
,
Sigma-Aldrich
), N-terminal anti-ERα (ab75635),
mouse
anti-KMT4/DOT1L
(
ab72454
), anti-
histone H3
(total;
ab1791
), anti-H3K79me1 (
ab2886
), anti-H3k79me2 (
ab3594
), anti-H3K79me3 (
ab2621
),
(9)
Nuclear lactate dehydrogenase A senses ROS to produce α-hydroxybutyrate for HPV-induced cervical tumor growth
Nature communications
October 24, 2018
Liu Y, Guo JZ, Liu Y, Wang K, Ding W, Wang H, Liu X, Zhou S, Lu XC, Yang HB,
et al
.
dilution), Histone H3 (
Cell Signaling Technology
,
4499
, with
1:5000
working dilution), DOT1L (
Abcam
,
ab72454
, with
1:1000
working dilution), NRF2 (
Abcam
,
ab62352
, with
1:1000
working dilution), SOD1(
Abcam
,
ab13498
,
(10)
C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation
Nature communications
April 30, 2018
Liu D, Zhang XX, Li MC, Cao CH, Wan DY, Xi BX, Tan JH, Wang J, Yang ZY, Feng XX,
et al
.
anti-H3K79me2/me3 (
ab2621
;
Abcam
), anti-H4K20me3 (
ab9053
;
Abcam
), anti-C/EBPβ (
ab18336
;
Abcam
), and anti-DOT1L (
ab72454
;
Abcam
). Primary antibodies
used in western blot
and IHC
were as follows: anti-C/EBPβ (
SAB4500112
;
Sigma
,
(11)
The protective role of DOT1L in UV-induced melanomagenesis
Nature communications
January 17, 2018
Zhu B, Chen S, Wang H, Yin C, Han C, Peng C, Liu Z, Wan L, Zhang X, Zhang J,
et al
.
anti-
XPC
(D1M5Y) (
1:1000
, #
14768
,
Cell Signaling Technology
). The Polyclonal
anti-KMT4/DOT1L
(
1:2000
,
ab72454
,
Abcam
) antibody, anti-XPA (
1:2000
,
ab85914
,
Abcam
), polyclonal di-methylated H3K79 antibody (
1:1000
,
(12)
IL-22(+)CD4(+) T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L
Immunity
May 15, 2014
Ilona Kryczek et al.
acetyl-
Histone H3
(
06-599
,
Millipore
),
Histone H3
(
9715
,
Cell Signaling
), β-Actin (
A5441
,
Sigma
), Dot1L (
ab72454
,
Abcam
), and Cleaved Notch1 (NICD,
ab52301
,
Abcam
). Signals were detected by ECL reagents (GE Healthcare,
(13)
Molecular mechanisms of selective estrogen receptor modulator activity in human breast cancer cells: identification of novel nuclear cofactors of antiestrogen-ERα complexes by interaction proteomics
Journal of proteome research
January 4, 2013
Cirillo F, Nassa G, Tarallo R, Stellato C, De Filippo MR, Ambrosino C, Baumann M, Nyman TA, Weisz A.
(14)
Human Polymerase-Associated Factor complex (PAFc) connects the Super Elongation Complex (SEC) to RNA polymerase II on chromatin
Proceedings of the National Academy of Sciences
September 6, 2011
Nanhai He, Caleb K. Chan, Bijan Sobhian, Seemay Chou, Yuhua Xue, Min Liu, Tom Alber, Monsef Benkirane, Qiang Zhou
and -
ELL2
(
A302-505A
-1) antibodies were purchased from
Bethyl Laboratories, Inc
.. The anti-Dot1L (
ab72454
) and anti-H3 dimethyl K79 (
ab3594
) antibodies were purchased from
Abcam
. The antibodies against CDK9
(15)
The leukemogenic AF4-MLL fusion protein causes P-TEFb kinase activation and altered epigenetic signatures
Leukemia
January 1, 2011
Benedikt A, Baltruschat S, Scholz B, Bursen A, Arrey TN, Meyer B, Varagnolo L, Müller AM, Karas M, Dingermann T,
et al
.
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Reviews
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Anti-KMT4 / Dot1L antibody from Abcam
Supplier Page
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Product Specs
Company Info
Citations
Reviews
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